rabbit anti human α 6 integrin subunit  (Santa Cruz Biotechnology)

Journal: Neuroscience Journal

Article Title: Roles of Integrins and Intracellular Molecules in the Migration and Neuritogenesis of Fetal Cortical Neurons: MEK Regulates Only the Neuritogenesis

doi: 10.1155/2013/859257

Figure Lengend Snippet: Antibodies, commercial sources, and applications.

Article Snippet: Monoclonal antibodies against α 6 integrin subunit (Clone NKI-GoH3) , BD Biosciences , Migration assays.

Techniques: Migration, Microscopy, Western Blot, Purification, Immunofluorescence

Journal: Neuroscience Journal

Article Title: Roles of Integrins and Intracellular Molecules in the Migration and Neuritogenesis of Fetal Cortical Neurons: MEK Regulates Only the Neuritogenesis

doi: 10.1155/2013/859257

Figure Lengend Snippet: Primers used for the amplification of integrin cDNA from rat fetal brain neurons.

Article Snippet: Monoclonal antibodies against α 6 integrin subunit (Clone NKI-GoH3) , BD Biosciences , Migration assays.

Techniques: Amplification

Journal: Neuroscience Journal

Article Title: Roles of Integrins and Intracellular Molecules in the Migration and Neuritogenesis of Fetal Cortical Neurons: MEK Regulates Only the Neuritogenesis

doi: 10.1155/2013/859257

Figure Lengend Snippet: Effects of antibodies, calcium modulators, and pharmacological inhibitors on neuritogenesis. (a) Representative images showing neuritogenesis in the absence (control) or presence of control IgG (50 nMole), antibodies against β 1 (50 or 100 nMole) or α 3 (50 or 100 nMole) integrin subunits on laminin. BAPTA-AM at low concentration (2.5 μ M) inhibited neuritogenesis and at higher concentration (10 μ M) totally abolished neuritogenesis. Bar150 microns. (b) Mean + standard error of mean values of neurite lengths/image in untreated neurons (1) and those treated with control IgG, monoclonal antibody against β 1 or α 3 integrin subunit at 50 nMole or 100 nMole concentration. Neurite lengths reduced significantly ( * P < 0.05) in neurons treated with antibody against β 1 integrin subunit at both 50 nMole (2) and 100 nMole (3) concentrations compared to control (1). Neurons treated with monoclonal antibody against α 3 integrin subunit at 50 nMole (4) were lower than the control (1) but was not statistically significant ( P > 0.05). Neurons treated with monoclonal antibody against α 3 integrin subunit at 100 nMole (5) significantly inhibited the neuritogenesis ( * P < 0.055). Neuritogenesis was not altered in presence of 50 (6) or 100 nMole (7) control IgG ( P > 0.05). (c) Mean + standard error of mean values of neurite lengths/image of neurons treated with PP2, PP3, U2, U3, 2-APB, activated Calphostin, BAPTA-AM (2.5 μ M), and PD for 22 h in culture (filled bars) were significantly different ( * P < 0.05) from untreated neurons (blank bar) and negative controls (PP3 or U3) (filled bars). No significant changes in neurite lengths ( P > 0.05) per image were recorded in neurons treated with RR.

Article Snippet: Monoclonal antibodies against α 6 integrin subunit (Clone NKI-GoH3) , BD Biosciences , Migration assays.

Techniques: Concentration Assay

Journal: Neuroscience Journal

Article Title: Roles of Integrins and Intracellular Molecules in the Migration and Neuritogenesis of Fetal Cortical Neurons: MEK Regulates Only the Neuritogenesis

doi: 10.1155/2013/859257

Figure Lengend Snippet: Transcripts of integrin subunits in the fetal cortical neurons. Ethidium bromide stained PCR products after agarose gel electrophoresis. Target mRNA species are shown at the bottom of the image. The 600 bp band of the 100 bp marker (M) is shown by arrows heads on sides.

Article Snippet: Monoclonal antibodies against α 6 integrin subunit (Clone NKI-GoH3) , BD Biosciences , Migration assays.

Techniques: Staining, Agarose Gel Electrophoresis, Marker

Journal: Neuroscience Journal

Article Title: Roles of Integrins and Intracellular Molecules in the Migration and Neuritogenesis of Fetal Cortical Neurons: MEK Regulates Only the Neuritogenesis

doi: 10.1155/2013/859257

Figure Lengend Snippet: Effects of antibodies on the migration of fetal cortical neurons. Monoclonal antibodies (shown below) against β 1 and α 3 integrin subunits significantly ( * P < 0.05) inhibited the migration of neurons (Neurons/field) on membranes coated with laminin (10 μ g/mL) (a). The migrations of neurons on laminin-coated membranes were not significantly ( P > 0.05) altered by antibody against α 6 or αv subunit (the negative control) and control antibodies (IgG or IgM). The migrations of neurons on fibronectin (100 μ g/mL) coated membranes were significantly ( * P < 0.05) inhibited by the antibody against β 1 integrin subunit only (b). The migrations of neurons on fibronectin-coated membranes were not altered by control antibodies (IgG or IgM) at P > 0.05.

Article Snippet: Monoclonal antibodies against α 6 integrin subunit (Clone NKI-GoH3) , BD Biosciences , Migration assays.

Techniques: Migration, Negative Control

Journal: Neuroscience Journal

Article Title: Roles of Integrins and Intracellular Molecules in the Migration and Neuritogenesis of Fetal Cortical Neurons: MEK Regulates Only the Neuritogenesis

doi: 10.1155/2013/859257

Figure Lengend Snippet: Schematic of integrin signaling cascade and its perturbation with antibodies and pharmacological agents. Integrin subunits (red and green bars on top) are shown intercalated in the membrane and interacting with ECM molecule (red and green horizontal lines). Molecules involved in signaling events are labeled and arrows point to the directions of signaling that starts with the engagement of integrin subunit with the extracellular matrix. Directions of calcium mediated signaling are shown by dashed green arrows. Inhibitors (see ) used for blocking signaling molecules and paths are shown by red blocks. Cross-talk between integrin and GF signaling is shown by double headed horizontal arrow close to membrane. Mab: monoclonal antibody, GF: growth factor, RTK: receptor tyrosine kinase, MEK: MAP kinase kinase, PLC: phospholipase C, PIP2: phosphotidal inositol biphosphate, IP3: inositol (1,4,5)-triphosphate (IP3), DAG: diacylglycerol and PKC: protein kinase C, Ras: G protein, Raf: MAPKKK, MEK: MAPKK, ERK: extracellular signal regulated kinase (MAPK).

Article Snippet: Monoclonal antibodies against α 6 integrin subunit (Clone NKI-GoH3) , BD Biosciences , Migration assays.

Techniques: Labeling, Blocking Assay

Journal: British Journal of Cancer

Article Title: Tetraspanin CD151 is a novel prognostic marker in poor outcome endometrial cancer

doi: 10.1038/bjc.2011.80

Figure Lengend Snippet: Immunohistochemistry with antibodies to CD151, HER-2, integrins α 6 β 1, α 3 β 1 and E-cadherin. A and B – CD151 ( × 100 and × 200, respectively); C and D – E-cadherin ( × 100 and × 200, respectively); E and F – integrin α 3 β 1 ( × 100 and × 200, respectively); G and H – integrin α 6 β 1 ( × 100 and × 200, respectively).

Article Snippet: Cell surface adhesion molecules goat anti-human α 3 integrin subunit (Santa Cruz, Santa Cruz, CA, USA, sc-6588, antibody concentration 0.67 μ g ml −1 ; dilution 1 : 300), rabbit anti-human α 6 integrin subunit (Santa Cruz, sc-10730, antibody concentration 0.5 μ g ml −1 ; dilution 1 : 400) and mouse anti-E-cadherin (clone NCH-38, Dako (Copenhagen, Denmark), antibody concentration 0.1 μ g ml −1 ; dilution 1 : 2000) were used.

Techniques: Immunohistochemistry